EV Core Sample Information Sheet

CUSTOMER INFORMATION:

Researcher's name and email:

Project ID/Title:

Project leader name and email:

Affiliation:

Contact email(s) for questions:

Email(s) for results:

Number of samples:

ORDER OF SERVICES (check boxes according to your selection)

EV isolation (no service right now)

Nanoparticle tracking analysis (NTA)

Apogee micro flow cytometry (no service right now)

Exoview (no service right now)

Spectradyne (no service right now)

Hematology analyzer

Custom services, which?

If yes, which?

Invoice information

Invoice information (HY:H-code and WBS):

Reference:

I accept the terms in this agreement and take fully responsibility of the costs of this research

I will acknowledge EV Core in my publications presenting EV Core's work carried out with the help of EV Core's resources (for academic research only)

I will notify the EV Core of every publication mentioning the EV Core

Sample type or source of EVs/particles:

Source of samples:

Sample buffer composition:

The number of samples:

The volume of sample:

Sample storage:

We cannot handle pathogenic, infectious or toxic samples.

If neutralized, explain:

Other security risks to be considered?

If yes, explain:

If possible please give estimate particle concentration for faster measurement.

What?

I need EV samples back after processing.

Project information

Description of project including details of the experiment.

Note our recommendations for sample preparation:

1. All samples: When possible, process and store samples according to the recommendations of the latest MISEV. Minimally, we recommend removal of cells (including platelets) and other larger non-EV bodies before freezing at -80C.

2. Samples for EV isolation: Consult the EV Core staff for sample types and required starting volumes for your chosen downstream method.

3. Nanoparticle tracking analysis (NTA) and Apogee micro flow cytometry:

A. Use filtered (0.1-0.2 µm) buffer and protein LoBind tubes for EV storage.

B. Aim at a high initial EV concentration and aliquot your sample to avoid freeze-thaw cycles.

C. For freezing, use -80C and deliver samples on dry ice to the EV Core.

For Zetaview NTA: By default three individual dilutions are prepared per sample. Each dilution is injected separately and measured with three measurement cycles. If this is not feasible for your samples, please contact EV Core for more information.

EV Core Viikki:

General project info

Pia Siljander

pia.siljander@helsinki.fi

Lab personnel

Rosabella Hartman

rosabella.hartman@helsinki.fi